This study examines the effect of hormone and amino acid levels on mood changes in women at mid-life. The research involved both a clinical trial of the application of hormones and amino acids to effect mood changes is women at mid-life, and a laboratory analysis of synthetic and natural progesterones. The clinical trial involved a detailed biochemical study of two women and a less intensive study of two other groups of women identified as Estrogen Dominant or Estrogen Deficient.